Co-exposure of polystyrene nanoplastics and copper induces development toxicity and intestinal mitochondrial dysfunction in vivo and in vitro.

Nanoplastics (NPs) have raised concerns about the combined toxicity to living organisms due to their ability to adsorb heavy metals. There is still uncertainty, however, whether NPs combined with heavy metals exert adverse effects on intestinal microenvironment, especially the intestinal cells and microbiota. Herein, the combined effects of 500 nm spherical-shaped polystyrene nanoplastics (PSNPs) and copper ions (Cu2+) on intestinal cells and gut microbiota were assessed using HCT-116 cells and zebrafish models. The combined exposure of PSNPs (10 mg/L) and Cu2+ (0.5 mg/L) induced more severer hatching interference of zebrafish embryos, deformation, and mortality. In larval stage, PSNPs (10 mg/L) accumulated and carried more Cu2+ in the gastrointestinal tract (GIT) of zebrafish after co-exposure for 5 days. Excessive neutrophil recruitment and oxidative stress in GIT of zebrafish larvae were observed. The mechanism of the combined toxicity was revealed by transmission electron microscopy (TEM) showing the injuries of GIT, transcriptome and 16S rDNA gene sequencing showing the toxicity pathways, including oxidative phosphorylation and respiratory electron transport chain, as well as microbial community analysis showing the induced microbiota dysbiosis. In vitro tests using HCT-116 cells showed that PSNPs (10 mg/L) and Cu2+ (0.5 mg/L) increased cell death while decreasing ATP concentration and mitochondrial membrane potential after 48 h exposure. These findings may provide new insights into the combined toxicity of nanoplastics and heavy metals in the intestinal microenvironment.

Identification of sedative-hypnotic compounds shared by five medicinal Polyporales mushrooms using UPLC-Q-TOF-MS/MS-based untargeted metabolomics.

BACKGROUND: Five Polyporales mushrooms, namely Amauroderma rugosum, Ganoderma lucidum, G. resinaceum, G. sinense and Trametes versicolor, are commonly used in China for managing insomnia. However, their active components for this application are not fully understood, restricting their universal recognition. PURPOSE: In this study, we aimed to identify sedative-hypnotic compounds shared by these five Polyporales mushrooms. STUDY DESIGN AND METHODS: A UPLC-Q-TOF-MS/MS-based untargeted metabolomics, including OPLS-DA (orthogonal projection of potential structure discriminant analysis) and OPLS (orthogonal projections to latent structures) analysis together with mouse assays, were used to identify the main sedative-hypnotic compounds shared by the five Polyporales mushrooms. A pentobarbital sodium-induced sleeping model was used to investigate the sedative-hypnotic effects of the five mushrooms and their sedative-hypnotic compounds. RESULTS: Ninety-two shared compounds in the five mushrooms were identified. Mouse assays showed that these mushrooms exerted sedative-hypnotic effects, with different potencies. Six triterpenes [four ganoderic acids (B, C1, F and H) and two ganoderenic acids (A and D)] were found to be the main sedative-hypnotic compounds shared by the five mushrooms. CONCLUSION: We for the first time found that these six triterpenes contribute to the sedative-hypnotic ability of the five mushrooms. Our novel findings provide pharmacological and chemical justifications for the use of the five medicinal mushrooms in managing insomnia.

Hybrid radical coupling during MnO2-mediated transformation of a mixture of organic UV filters: Chemistry and toxicity assessment.

Manganese oxide (MnO2) is one of the most abundant metal oxides, and it is renowned for its ability to degrade various phenolic micropollutants. However, under MnO2-mediated transformation, BP-3 transforms into 12 different radical-coupled transformation products (TPs) out of 15 identified TPs. These radical-coupled TPs are reported with adverse environmental impacts. This study explored the effects of MnO2 on organic UV filter mixtures and different water constituents (i.e., bicarbonate ion (HCO3-), humic acid (HA) and halide ions) in terms of degradation efficiency and transformation chemistry. When a mixture of benzophenone-3 (BP-3) and avobenzone (AVO) underwent transformation by MnO2, hybrid radical-coupled TPs derived from both organic UV filters were generated. These hybrid radical-coupled TPs were evaluated by an in silico prediction tool and Vibrio fischeri bioluminescence inhibition assay (VFBIA). Results showed that these TPs were potentially toxic to aquatic organisms, even more so than their parent compounds. The higher the concentration of HCO3-, HA, chloride ion (Cl-) and bromide ion (Br-), the greater the reduction in the efficiencies of degrading BP-3 and AVO. Contrastingly, in the presence of iodide ion (I-), degradation efficiencies of BP-3 and AVO were enhanced; however, iodinated TPs and iodinated radical-coupled TPs were formed, with questionable toxicity. This study has revealed the environmental risks of hybrid radical-coupled TPs, iodinated TPs and iodinated radical-coupled TPs when the organic UV filters BP-3 and AVO are transformed by MnO2.

Co-exposure to organic UV filters and phthalates and their associations with oxidative stress levels in children: A prospective follow-up study in China.

Children are highly vulnerable to environmental pollutants, especially endocrine-disrupting chemicals (EDCs). Previous research has linked both organic UV filters and phthalates exposure to adiposity and pubertal development in children. Nevertheless, the individual and collective effects of these chemicals on this population remain poorly understood. In this study, twelve organic UV filters and metabolites, six phthalate metabolites and two oxidative stress biomarkers were analyzed in a prospective follow-up study in Shanghai, China after a baseline study conducted 1.5 years earlier. Results revealed a positive association between exposure to individual organic UV filters or their mixture and levels of 8-OHdG (ß ranging from 0.242 to 0.588, P < 0.05), a marker of oxidative DNA damage. BP-3 and OD-PABA made a greater contribution to oxidative DNA damage than other UV filters. Levels of 8-OHdG were also positively correlated with single phthalate metabolites and their mixture, with MnBP and MMP contributing the most. Stratified analysis found that these associations were mainly observed in girls. Our mixture analysis revealed cumulative risks of oxidative DNA damage when there was co-exposure to these two kinds of EDCs. These results underscore the importance of considering the risks associated with organic UV filters and the necessity of evaluating the effects of all these pollutants, both individually and in mixtures.

Hazardous radical-coupled transformation products of benzophenone-3 formed during manganese dioxide treatment.

Radical-coupled transformation products (TPs) have been identified as the byproducts of various transformation processes, including both natural attenuation and artificial treatments, of phenolic micropollutants. Benzophenone-3 (BP-3), an organic UV filter of emerging concern, has been previously reported with ubiquitous occurrence in the natural environment and water bodies. Current research has demonstrated how TPs are formed from BP-3 when it is treated with manganese oxide (MnO2). The ecological and toxicological risks of these TPs have also been assessed. Polymerization of BP-3 through radical coupling was observed as the major pathway by which BP-3 is transformed when treated with MnO2. These radical-coupled TPs haven't shown further degradation after formation, suggesting their potential persistence once occurred in the environment. In silico experiments predict the radical-coupled TPs will increase in mobility, persistence and ecotoxicity. If true, they also represent an ever-increasing threat to the environment, ecosystems and, most immediately, aquatic living organisms. In addition, radical-coupled TPs produced by MnO2 transformation of BP-3 have shown escalated estrogenic activity compared to the parent compound. This suggests that radical coupling amplifies the toxicological impacts of parent compound. These results provide strong evidence that radical-coupled TPs with larger molecular sizes are having potential adverse impacts on the ecosystem and biota.

Chlorination of bisphenols in water: Understanding the kinetics and formation mechanism of 2-butene-1,4-dial and analogues.

While it is widely accepted that 2-butene-1,4-dial (BDA) is a toxic metabolite with genotoxic and carcinogenic properties, little is known about BDA and its analogues (BDAs) formation during water disinfection. In this study, the effects of different chlorination conditions on the formation of BDAs from bisphenol and its analogues (BPs analogues) were evaluated. A transformation pathway for the formation of BDAs upon chlorination of BPs analogues is proposed. The time profile of the transformation of BPs analogues into BDAs reveals that the generation of dichlorohydroquinone, dichloro-hydroxybenzenesulfonic acid and 2,4,6-trichlorophenol, are significantly associated with the formation of BDAs in the disinfected water. Owing to the different bridging groups contributing to the electrophilicity of BPs analogues in varying degrees, the stronger the electrophilicity of BPs analogues the more BDAs are formed. In addition, the type of BDAs produced is also affected. Four types of BDAs were detected in this study, one of which was newly identified. This study confirms that BPs analogues are an important source of BDAs and provides more insights into the formation of BDAs during chlorination. Greater attention should be given to the formation of BDAs in chlorinated water and their potential threat to humans and the ecosystem.

A tri-herb formulation protects against ethanol-induced mouse liver injury and downregulates mitogen-activated protein kinase phosphatase 1.

BACKGROUND: A tri-herb formulation comprising Ganoderma (the dried fruiting body of Ganoderma lucidum), Puerariae Thomsonii Radix (the dried root of Pueraria thomsonii) and Hoveniae Semen (the dried mature seed of Hovenia acerba) -GPH for short- has been using for treating liver injury; however, the pharmacological basis of this application of GPH is unknown. This study aimed to investigate the liver protective effects and mechanisms of action of an ethanolic extract of GPH (GPHE) in mice. METHODS: To control the quality of GPHE, the contents of ganodermanontriol, puerarin and kaempferol in the extract were quantified by ultra-performance liquid chromatography. An ethanol (6 ml/kg, i.g.)-induced liver injury ICR mouse model was employed to investigate the hepatoprotective effects of GPHE. RNA-sequencing analysis and bioassays were performed to reveal the mechanisms of action of GPHE. RESULTS: The contents of ganodermanontriol, puerarin and kaempferol in GPHE were 0.0632%, 3.627% and 0.0149%, respectively. Daily i.g. administration of 0.25, 0.5 or 1 g/kg of GPHE for 15 consecutive days suppressed ethanol (6 ml/kg, i.g., at day 15)-induced upregulation of serum AST and ALT levels and improved histological conditions in mouse livers, indicating that GPHE protects mice from ethanol-induced liver injury. Mechanistically, GPHE downregulated the mRNA level of Dusp1 (encoding MKP1 protein, an inhibitor of the mitogen-activated protein kinases JNK, p38 and ERK), and upregulated expression and phosphorylation of JNK, p38 and ERK, which are involved in cell survival in mouse liver tissues. Also, GPHE increased PCNA (a cell proliferation marker) expression and reduced TUNEL-positive (apoptotic) cells in mouse livers. CONCLUSION: GPHE protects against ethanol-induced liver injury, and this effect of GPHE is associated with regulation of the MKP1/MAPK pathway. This study provides pharmacological justifications for the use of GPH in treating liver injury, and suggests that GPHE has potential to be developed into a modern medication for managing liver injury.

A machine learning approach for early prediction of gestational diabetes mellitus using elemental contents in fingernails.

The aim of this pilot study was to predict the risk of gestational diabetes mellitus (GDM) by the elemental content in fingernails and urine with machine learning analysis. Sixty seven pregnant women (34 control and 33 GDM patient) were included. Fingernails and urine were collected in the first and second trimesters, respectively. The concentrations of elements were determined by inductively coupled plasma-mass spectrometry. Logistic regression model was applied to estimate the adjusted odd ratios and 95% confidence intervals. The predictive performances of multiple machine learning algorithms were evaluated, and an ensemble model was built to predict the risk for GDM based on the elemental contents in the fingernails. Beryllium, selenium, tin and copper were positively associated with the risk of GDM while nickel and mercury showed opposite result. The trained ensemble model showed larger area under curve (AUC) of receiver operating characteristic curve (0.81) using fingernail Ni, Cu and Se concentrations. The model was validated by external data set with AUC = 0.71. In summary, the results of the present study highlight the potential of fingernails, as an alternative sample, together with machine learning in human biomonitoring studies.

In situ turning defects of exfoliated Ti3C2 MXene into Fenton-like catalytic active sites.

Controllable in situ formation of nanoclusters with discrete active sites is highly desirable in heterogeneous catalysis. Herein, a titanium oxide-based Fenton-like catalyst is constructed using exfoliated Ti3C2 MXene as a template. Theoretical calculations reveal that a redox reaction between the surface Ti-deficit vacancies of the exfoliated Ti3C2 MXene and H2O2 molecules facilitates the in situ conversion of surface defects into titanium oxide nanoclusters anchoring on amorphous carbon (TiOx@C). The presence of mixed-valence Tiδ+ (δ = 0, 2, 3, and 4) within TiOx@C is confirmed by X-ray photoelectron spectroscopy (XPS) and X-ray absorption fine structure (XAFS) characterizations. The abundant surface defects within TiOx@C effectively promote the generation of reactive oxygen species (ROS) leading to superior and stable Fenton-like catalytic degradation of atrazine, a typical agricultural herbicide. Such an in situ construction of Fenton-like catalysts through defect engineering also applies to other MXene family materials, such as V2C and Nb2C.

The quest for metabolic biomarkers of agrochemicals exposure via in vitro studies and suspect screening.

Numerous agrochemicals, including pesticides and herbicides, are applied in modern agriculture, resulting in concerns for the ecosystem and human safety as humans are easily exposed to these compounds. Many agrochemicals, and their transformation products or metabolites, have shown toxicity in in vitro and in vivo studies. However, given the rapid development of novel agrochemicals, for many there is no information about their effects nor about metabolic transformations when ingested by humans. Tracing biomarkers may be the best method for assessing the impacts of agrochemicals. A combination of in vitro metabolism study and suspect screening of human samples (e.g., urine, blood) can be utilized to efficiently find biomarkers for agrochemical exposure. In the work reported here, we determined the in vitro metabolic profiling of six prioritized pesticides and synergists, namely boscalid, carbendazim, piperonyl butoxide, spiroxamine, dimethomorph and fludioxonil, in human liver microsomes. 17 major metabolites were structurally elucidated by high resolution mass spectrometry (HRMS). Major metabolic transformation processes (e.g., hydroxylation, demethylation and oxidation) were proposed for each pesticide. Individual in silico toxicity assessments showed that some metabolites had the same or even enhanced toxicity compared to parent compounds. Information about these metabolites obtained from HRMS was used for suspect screening in human activities related samples. Carbendazim and a metabolite of fludioxonil were identified in wastewater and laboratory urine samples, respectively. Our findings provide concrete evidence for the use of in vitro metabolites as biomarkers in biomonitoring studies of potential exposure to toxic chemicals.

Suppression of spectral interference in dual-elemental analysis of single particles using triple quadrupole ICP-MS.

Single particle-inductively coupled plasma-mass spectrometry (SP-ICP-MS) was used in the analysis of single particles/cells. Although quadrupole mass analyzers are widely used, the long settling time restricts measurement to single elements in individual particles. Recently, dual-elemental analysis has successfully been developed with the assistance of oxygen gas in the collision cell. This simple approach greatly expands the capability of quadrupole-based ICP-MS. In this study, we adopted bandpass mode in the first quadrupole (Q1) to improve the limit of detection of single particles against spectral interference. A model was developed to explain the rationale behind the selection of quadrupole voltages. The quadrupole voltages were optimized systematically so that the mass bandwidth of Q1 allowed the transmission of two target analytes while the interference species were rejected. As a result, the signal from the polyatomic interference was reduced by 98% with no significant change in the analyte signal. The bandpass mode was further applied to accurately determine the isotope ratio of 109Ag/107Ag in 80-nm Ag nanoparticles, as well as the Ag content in AgSn alloy particles, under the polyatomic interference of 91Zr16O originating from dissolved ions and particles. This technique was further applied to the determination of two Yb isotopes in algal cells with interference from Gd. Results indicate that this approach has great potential for assessing single particles and biological cells in the presence of severe interference from imaging agents, drugs, or biological fluids.

An integrated ICP-MS-based analytical approach to fractionate and characterize ionic and nanoparticulate Ce species.

Cerium dioxide nanoparticles (CeO2 NPs) are widely used in various fields, leading to concern about their effect on human health. When conducting in vivo investigations of CeO2 NPs, the challenge is to fractionate ionic Ce and CeO2 NPs and to characterize CeO2 NPs without changing their properties/state. To meet this challenge, we developed an integrated inductively coupled plasma-mass spectrometry (ICP-MS)-based analytical approach in which ultrafiltration is used to fractionate ionic and nanoparticulate Ce species while CeO2 NPs are characterized by single particle-ICP-MS (sp-ICP-MS). We used this technique to compare the effects of two sample pretreatment methods, alkaline and enzymatic pretreatments, on ionic Ce and CeO2 NPs. Results showed that enzymatic pretreatment was more efficient in extracting ionic Ce or CeO2 NPs from animal tissues. Moreover, results further showed that the properties/states of all ionic and nanoparticulate Ce species were well preserved. The rates of recovery of both species were over 85%; the size distribution of CeO2 NPs was comparable to that of original NPs. We then applied this analytical approach, including the enzymatic pretreatment and ICP-MS-based analytical techniques, to investigate the bioaccumulation and biotransformation of CeO2 NPs in mice. It was found that the thymus acts as a "holding station" in CeO2 NP translocation in vivo. CeO2 NP biotransformation was reported to be organ-specific. This is the first study to evaluate the impact of enzymatic and alkaline pretreatment on Ce species, namely ionic Ce and CeO2 NPs. This integrated ICP-MS-based analytical approach enables us to conduct in vivo biotransformation investigations of CeO2 NPs.

Dual-elemental analysis of single particles using quadrupole-based inductively coupled plasma-mass spectrometry.

Single-particle inductively coupled plasma-mass spectrometry (SP-ICP-MS) is used for elemental analysis of single particles and biological cells. Time-of-flight (TOF) mass analyzers are widely used for multiple element analysis of individual particles. Owing to the sequential nature of the mass analyzer, quadrupole-based ICP-MS generally gives poor analytical performance when more than one element are being monitored. In this study, we present the first accurate and precise dual-mass measurement of individual particles using quadrupole-based ICP-MS, with the assistance of oxygen collision gas. Simultaneous measurement of the intensity of 107Ag and 109Ag of Ag nanoparticles (AgNP) showed particle recovery of 100% and Pearson correlation coefficient of 0.97, indicating proper sampling of all particles in the ICP. This technique gives good measurement precision (RSD <8%) and high accuracy in size measurement (error <3%). This technique was further applied to determine the elemental content and isotope ratios of particles and to study cell viability after Cisplatin staining. The results are comparable to that of existing TOF and multi-collector ICP-MS, indicating that quadrupole-based ICP-MS can be a cost-effective alternative for simultaneous measurement of two isotopes. Acquisition with longer integration time and shorter settling time is also proposed to further improve the sensitivity and number of isotopes monitored. This study will potentially open up more possibilities of using quadrupole based ICP-MS in biomedical research as quantification of multi-elements in single cells is far more informative. Other possible applications include classification of cancer subtypes according to the abundance of several biomarkers, as well as elemental bio-imaging of transcripts and proteins in tissues by laser ablation.

Current applications and future perspectives on elemental analysis of non-invasive samples for human biomonitoring.

Humans are continuously exposed to numerous environmental pollutants including potentially toxic elements. Essential elements play an important role in human health. Abnormal elemental levels in the body, in different forms that existed, have been reported to be correlated with different diseases and environmental exposure. Blood is the conventional biological sample used in human biomonitoring. However, blood samples can only reflect short-term exposure and require invasive sampling, which poses infection risk to individuals. In recent years, the number of research evaluating the effectiveness of non-invasive samples (hair, nails, urine, meconium, breast milk, placenta, cord blood, saliva and teeth) for human biomonitoring is increasing. These samples can be collected easily and provide extra information in addition to blood analysis. Yet, the correlation between the elemental concentration in non-invasive samples and in blood is not well established, which hinders the application of those samples in routine human biomonitoring. This review aims at providing a fundamental overview of analytical methods of non-invasive samples in human biomonitoring. The content covers the sample collection and pretreatment, sample preparation and instrumental analysis. The technical discussions are separated into solution analysis and solid analysis. In the last section, the authors highlight some of the perspectives on the future of elemental analysis in human biomonitoring.

Comparative physicochemical properties and toxicity of organic UV filters and their photocatalytic transformation products.

Transformation products (TPs) of micropollutants contaminating our water resources have become an emerging issue due to the potential threats they pose to environmental and human health. This study investigated the transformation chemistry, toxicity, physicochemical properties and environmental behavior resulting from photocatalytic transformation of organic UV filters as model micropollutants. 3-Benzylidene camphor (3-BC), 4-hydroxybenzophenone (4-HB) and octocrylene (OC) were effectively degraded by UV-A/TiO2 treatment, with TPs identified and characterized with high resolution mass spectrometry. Nitrated-TPs were observed to be formed in the presence of nitrite and nitrate for 3-BC and 4-HB, suggesting that the transformation process could be altered by components in the water matrix. Vibrio fischeri bioluminescence inhibition assay revealed an increase in toxicity of TPs derived from photocatalytic treatment, with quantitative structure-activity relationship model (ECOSAR) predicted an enhanced toxicity of individual TPs' after transformation. Assessment of physicochemical properties and environmental behavior suggested that TPs as compared to parent organic UV filters, may represent even greater hazards due to their increased water solubility, persistence and mobility - in addition to retaining the parent organic UV filter's toxicity. The results provide important information relevant to the potential risks for the selected organic UV filters, and their corresponding transformation products.

Acesulfame aerobic biodegradation by enriched consortia and Chelatococcus spp.: Kinetics, transformation products, and genomic characterization.

The artificial sweetener Acesulfame (ACE) has been frequently detected in wastewater treatment plants (WWTPs) and is regarded as an emerging pollutant due to its low biodegradability. However, recent observations of ACE biodegradation in WWTPs have stimulated interest in the ACE-degrading bacteria and mineralization pathways. In this study, next-generation sequencing methods, Illumina and Nanopore sequencing, were combined to explore the ACE-degrading communities enriched from the activated sludge of six municipal wastewater treatment plants. Metagenomic investigations indicated that all enrichments were similarly dominated by the phyla Proteobacteria and Planctomycetes. Notably, at the species level, four metagenome-assembled genomes (MAGs) were shared by six enriched communities with considerable abundances, indicating that they may be responsible for ACE biodegradation in the enrichments. Besides, two ACE-degrading pure strains, affiliated to the genus Chelatococcus, were isolated from the enrichment. The genomic analysis showed that these two isolates were the new species that were genetically distinct from their relatives. Two type strains, Chelatococcus asaccharovorans DSM 6462 and Chelatococcus composti DSM 101465, could not degrade ACE, implying that the ACE-degrading capability was not shared among the different species in the genus Chelatococcus. The results of the degradation experiment showed that the two isolates could use ACE as the sole carbon source and mineralize ~90% of the total organic carbon. Three biotransformation products (TP96, TP180B, and TP182B) were demonstrated by UPLC-QTOF-MS. The results of this study provide valuable insights into ACE biodegradation and its biotransformation products.

The crucial role of heavy metals on the interaction of engineered nanoparticles with polystyrene microplastics.

Despite continuous research on microplastics (MPs), studies exploring the complexity of interaction between MPs and other aqueous constituents in multi-solute systems are scarce. In this study, the uptake and release of nanoceria (CeNPs) by various polystyrene MPs (PSMPs) were investigated. Results showed that PSMPs in the presence of heavy metals (HMs) exhibited a substantially higher sorption affinity for isotropic charged CeNPs than PSMPs alone; this enhanced affinity was attributed to the formation of PSMP-HM-CeNP complexes. FE-SEM imaging reaffirmed that CeNP clusters adhered to PSMP surfaces in the presence of HMs. Such attachment varied dependent on valence state, atomic size of coexisting metal cations, surface texture, and functionalities of MPs. The HM-mediated complex formation on PSMP particles was suppressed at higher ionic strength because of competitive sorption and double-layer compression. Subsequent release of MP-adhered CeNPs and HMs varied significantly between aquatic media and various simulated digestive fluids, verifying the crucial role of MPs for transfer of engineered nanoparticles (ENPs) from natural environments into biota via ingestion of MPs and trophic transfer. Our results highlight the enhanced potential for MPs to accumulate and to transport ENPs when metallic contaminants are present, which adds to the current understanding of the environmental fate and adverse effects of MPs along with various waterborne contaminants in actual environments.

Risks of organic UV filters: a review of environmental and human health concern studies.

Organic UV filters are compounds that absorb UV irradiation by their highly conjugated structure. With the developing consciousness over the last century of the skin damage UV radiation can cause, the demand for organic UV filters has risen, for use not only in sunscreens, but also in other personal care products. The massive production and usage of these organic UV filters has resulted in extensive release into the aquatic environment, and thereby making an important group of emerging contaminants. Considering the widespread occurrence of organic UV filters in not only ambient water, but also sediment, soil and even indoor dust, their threats towards the health of living organisms have been a subject of active investigation. In this review article, we present an overall review of existing knowledge on the risks of organic UV filters from the aspects of both environmental and human health impacts. As for the environment, some organic UV filters are proven to bioaccumulate in various kinds of aquatic organisms, and further to have adverse effects on different kinds of animal models. Toxicological studies including in vivo and in vitro studies are important and effective means to ascertain the effects and mechanisms of organic UV filters on both the ecosystem and humans. Subsequent concerns arise that these compounds will affect human health in the long term. This review concludes by suggesting future lines of research based on the remaining knowledge gaps.

Degradation of acesulfame in UV/monochloramine process: Kinetics, transformation pathways and toxicity assessment.

UV/monochloramine (UV/NH2Cl) is an emerging advanced oxidation process that can generate various reactive species like reactive chlorine species (RCS) and hydroxyl radicals for micropollutant removal. This study investigated the potential toxicity of transformation products resulting from UV/NH2Cl treatment of acesulfame (ACE), as an example of micropollutant, found in worldwide aquatic environment. Compared with UV photolysis and chloramination, the UV/NH2Cl process more effectively degraded ACE. The transformation products of ACE treated with the UV/NH2Cl process were identified and characterized with high resolution mass spectrometry. The formation of chlorinated-TPs indicated the role of RCS in UV/NH2Cl transformation even though UV photolysis was predominantly responsible for the ACE degradation. The Vibrio fischeri bioluminescence inhibition assay revealed a higher toxicity of TPs derived from UV/NH2Cl than from UV photolysis. The increased toxicity could be attributed to most of the generated chlorinated-TPs (Cl-TPs), in particular those halo-alcohols. The ECOSAR program predicts that halo-alcohol TPs are more toxic than their non-chlorinated analogues and other Cl-TPs. This study provides insight into the important role of reactive species in the micropollutants' transformation of UV/NH2Cl process. It further provides information relevant to the potential risk when applying the process for micropollutant removal in water treatment.

Effects of Weathering on the Sorption Behavior and Toxicity of Polystyrene Microplastics in Multi-solute Systems.

Recent studies have demonstrated that weathering modifies the physicochemical properties and sorption behavior of microplastics (MPs). However, little is known about the effects of such weathering on the simultaneous sorption by MPs of different organic pollutants in multi-solute systems. In this study, the role of cosolute properties in the formation of solute multilayers with a hydrophobic primary solute (4-MBC) on pristine and various weathered polystyrene MPs (PSMPs) was examined. Three weathered PSMPs were studied namely, UV-irradiated PS (UV-PS), microbially degraded PS (MD-NPS), and UV-irradiated PS with subsequent microbial degradation (MD-UV-PS). The weathered PSMPs generally exhibited higher degree of oxygenated functionalities with less surface hydrophobicity than pristine particles. Our findings showed that the formation of solute multilayers with hydrophobic cosolutes was drastically suppressed in UV-PS due to more severe competition at hydrophobic sorption sites. Nevertheless, hydrophilic cosolutes contributed to solute multilayer formation with 4-MBC on PSMPs after UV irradiation, probably due to the stronger sorption of hydrophilic compounds to the oxidized surfaces of these particles via enhanced H-bonding. Strikingly, the sorption of 4-MBC by MD-UV-PS was notably enhanced when hydrophobic cosolutes were present. The observed synergistic sorption indicates that adhered biofilms and/or organic matter on MD-UV-PS could sorb the hydrophobic cosolute molecules, and eventually promote sorption of 4-MBC. Our further toxicity tests revealed that such solute multilayers formed on PSMPs inhibited microalgal growth. These results suggest that the fate and biological effects of MP-mediated chemical exposure could be strongly affected by weathering processes and coexistence of multiple organic contaminants in natural environments.